study on dgat1-exon8 polymorphism in iranian buffalo

نویسندگان

deniz heydarian

seyed reza miraei-ashtiani

mostafa sadeghi

چکیده

objective: diacylglycerol acyltransferase 1 is a microsomal enzyme that catalyzes the final step of triglyceride synthesis. the objective of this project is to check out the polymorphism at the exon 8 region of dgat1 gene using pcr-sscp technique in iranian water buffaloes (bubalus bubalis). recent activities have shown that a significant association between lysine at amino acid position 232 with higher milk fat content, whereas an alanine at this position is marker data of lower milk fat content and higher milk yield. methods: in this study we analyzed buffaloes from 5 provinces of iran. three pcr-sscp patterns were found. results: dna sequencing analysis showed that all of the patterns had the motif “aa” at position 6962-6963 (k allele) encoding lysine, which is similar to “aa” at position 10433-10434 in bos indicus and also different buffalo breeds at position 6962-6963. therefore exon 8 is a conservative region in bos indicus and all buffalo breeds which is associated with higher milk fat content. moreover, we found a base transition at position 7036 c/t and we have heterozygosity of both “c and t”, which is located at intron 8 of dgat1 gene in few samples. conclusions: this transition is a nonsense mutation which causes no change in amino acid sequence. this mutation has occurred at the intron 8 of dgat1 gene in iranian buffaloes.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Study on DGAT1-exon8 Polymorphism in Iranian Buffalo

Objective: Diacylglycerol acyltransferase 1 is a microsomal enzyme that catalyzes the final step of triglyceride synthesis. The objective of this project is to check out the polymorphism at the exon 8 region of DGAT1 gene using PCR-SSCP technique in Iranian water buffaloes (Bubalus bubalis). Recent activities have shown that a significant association between lysine at amino acid positi...

متن کامل

Study on DGAT1-exon8 Polymorphism in Iranian Buffalo

Objective: Diacylglycerol acyltransferase 1 is a microsomal enzyme that catalyzes the final step of triglyceride synthesis. The objective of this project is to check out the polymorphism at the exon 8 region of DGAT1 gene using PCR-SSCP technique in Iranian water buffaloes (Bubalus bubalis). Recent activities have shown that a significant association between lysine at amino acid positi...

متن کامل

Polymorphism and Sequencing of DGAT1 Gene in Iranian Holstein Bulls

Quantitative traits locus for milk production traits has been described on centromeric end of bovine chromosome 14. Reports name the acyl coA: diacylglycerol acyltransferase (DGAT1) gene as a potential candidate gene with dinucleotide substitution (AA to GC) in exon VIII which causes the change of lysine to alanine in amino acid (K232A).The aim of the present study was to estimate the frequency...

متن کامل

DGAT1 and ABCG2 polymorphism in Indian cattle (Bos indicus) and buffalo (Bubalus bubalis) breeds

BACKGROUND Indian cattle (Bos indicus) and riverine buffalo (Bubalus bubalis) give a poor yield of milk but it has a high fat and protein percentage compared to taurine cattle. The identification of QTLs (Quantitative Trait Loci) on BTA14 and BTA6 and its subsequent fine mapping has led to identification of two non conservative mutations affecting milk production and composition. Our objective ...

متن کامل

reflections on taught courses of the iranian ma program in english translation: a mixed-methods study

the issue of curriculum and syllabus evaluation and revision has been in center of attention right from when curriculum came into attention of educational institutions. thus everywhere in the world in educational institutions curricula and syllabi are evaluated and revised based on the goals, the needs, existing content, etc.. in iran any curriculum is designed in a committee of specialists and...

Polymorphism of SCD1 and DGAT1 Gene in Isfahan Holstein Cows

In this study we estimated the allele and genotype frequencies of SCD1 and DGAT1 gene polymorphism. The analysis was conducted on 408 Holstein cows from five dairy herds in Isfahan province. Genotypes were identified using PCR-RFLP technique. Two genetic variants (A and V) of the SCD1 gene were observed in this experiment. The frequency of A-allele ranged from 0.49 to 0.66, while frequency of V...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
international journal of advanced biological and biomedical research

ناشر: casrp publishing company

ISSN 2383-2762

دوره 2

شماره 7 2014

کلمات کلیدی

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023